The Protein Crystallography Beamline at CAMD |
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August, 2007 -- Installation of Biolab nears completionA biolab had been constructed adjacent to the beamline. The biolab is available for beamline staff and users to conduct crystallography related experiments. In addition to common lab equipment, the biolab also contains a CoyLab anaerobic chamber for the preparation of oxygen sensitive samples. January, 2005 -- Cold Room InstalledA small 4° C cold room has been installed at the beamline. This cold room will be available for use by users of the beamline to store crystals or other supplies while working at the beamline. The cold room is part a the larger effort to build a small biolab that will enable structural biology research to be conducted on site at CAMD. January, 2005 -- Multilayer Monochromator InstalledThe new multilayer monochromator has been installed by Oxford Danfysik. In the coming months the monochromator will undergo commissioning by the beamline staff. During the commissioning process the beamline staff will explore the capabilities of the monochromator in order to better advise beamline users as to when use of the multilayer monochromator will benefit their experiments. October 19, 2004 -- New Multilayer Monochromator ArrivedIn order to provide higher flux for non-MAD data collection, we have purchased a new multilayer monochromator. The multilayer system will have a bandpass of about 0.5% compared to 0.02% for the present Si (111) monochromator. The higher bandpass is expected to produce a several fold increase in the X-ray flux of the beamline at the sample. The installation of the multilayer monochromator was designed such that it and the Si (111) monochromator can be rapidly interchanged depending on the requirements of the experiment. The present Si (111) monochromator will be used for MAD data collection and other experiments requiring the narrower bandpass, while the multilayer monochromator will be available for data collections where the higher flux is desired and when the wider bandpass would not adversely affect the experiment (for example single wavelength data collections). October 1, 2004 -- Assistant Beamline Manager HiredDr. David Neau has been hired as the Assistant Beamline Manager. David comes from Jeff Bolin's lab at Purdue University where he recently completed his PhD in Structural Biology. In his role as Assistant Beamline Manager, David will help maintain and operate the GCPCC protein crystallography beamline, assist users at the beamline, and help facilitate access to the beamline by general users in order to fulfill the commitment of 25% general use. January 7, 2003 -- Beamline Opened for User OperationThe GCPCC protein crystallography beamline is now open for user access. Groups wishing to schedule beam time at the beamline should contact the beamline staff (gcpcc_access@xray.utmb.edu). The current beam status and the schedule for the next several months can be found at http://camd.lsu.edu/schedule_beam/beamschedule.htm. In general, the GCPCC beamline will be running whenever there is beam in the ring. March 15, 2002 -- Detector Received and InstalledThe MAR Research 165 mm dia. CCD detector and MAR Research dtb (desk top beamline) goniostat have been installed in the user hutch and tested with synchrotron radiation. The goniostat is highly automated and incorporates a 2-theta motion for high resolution data collection and a chi motion for mounting pre-frozen crystals directly from the cryovial. The CCD system features a low noise detector with 3.5 sec. readout time. For details see the MAR Research products web page. February 15, 2002 -- First LightSynchrotron light was brought down the beamline and into the user hutch for the first time. The beamline optical elements were aligned to the beam and the monochromator was calibrated. Tests showed that the bandpass of the monochromator light was narrow enough for MAD crystallography. Dr. Peter Siddons from the National Synchrotron Light Source came to CAMD to assist in commissioning the beamline optics. July 9, 2001 -- Beamline Manager HiredDr. Henry Bellamy has been hired as the Beamline Manager, and will start in his new position at the end of September. Henry has been at the Stanford Synchrotron Radiation Laboratory (SSRL) since 1988, where he is in charge of beamline 1-5. SSRL beamline 1-5 is a MAD beamline like the new GCPCC protein crystallography beamline. He will supervise the completion of the experimental hutch and user area as well as the commissioning of the beamline and data collection equipment. Henry has a Ph.D. in Molecular Biology from Washington University in St. Louis. His research interests involve the study of crystal perfection by means of high resolution reflection profile analysis. January 21, 2000 -- Beamline Construction in ProgressAfter completing a detailed ray-tracing study of our beamline design, we developed bid specifications that would allow us to realize our optical design with a single vendor beamline solution. Prior to releasing the bid specifications, we solicited feedback from external scientists about our detailed beamline plans. The invitation to bid was released by the LSU purchasing department on December 20, 1999 and the bids were opened on January 19, 2000 . Oxford Instruments Accelerator Technology Group (now Oxford-Danfysik) was the successful bidder for the beamline. This order includes two 1.4 m long rhodium coated mirror systems, a channel-cut Si (111) monochromator, diagnostic screens, and beam defining apertures. September 15, 1999 -- Funding Approval for Beamline ReceivedThe National Science Foundation (NSF) Division of Biological Infrastructure (DBI) has awarded funding for the Gulf Coast Protein Crystallography Consortium's (GCPCC) beamline development. The National Institutes of General Medical Sciences (NIGMS) at the National Institutes of Health (NIH) is also contributing to the project by providing matching funds through an interagency agreement with the NSF. Matching funds are also being provided by the institutions participating in the GCPCC. The NSF award number is DBI-9871464. |