The Protein Crystallography Beamline at CAMD

 

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The GCPCC beamline is located at the Center for Advanced Microstructures and Devices (CAMD), which is operated by Louisiana State University.  The CAMD synchrotron is a second generation source that is currently operated at 1.3 GeV with an injection current of 100 mA and 2 fills per day.

The GCPCC beamline utilizes 1.5 mrad of the output of a 11-pole 7.5 Tesla super conducting wiggler. The x-rays are collimated vertically by a fixed radius cylindrical mirror, monochromatized by either a channel-cut monochromator or a multilayer monochromator and focused by a toroidal mirror with an adjustable major radius.

The channel-cut monochromator is designed for MAD data collection and  has a channel-cut Si crystal in the 111 orientation.  The bandpass (Δλ/λ) is approximately 2x10-4.  The multilayer monochromator will have a bandpass of about 5x10-3 which will greatly increase the x-ray flux on the sample.   The bandwidth from the multilayer system will be too large for MAD use but will be good for most other data collection from crystals with a unit cell of less than about 200 Å.   It is possible to switch between the 2 monochromators depending on the requirements of the experiment.


 

Source

  • Ring energy: 1.3 GeV
  • Ring current (at injection): 100 mA - 2 fills per day
  • Insertion Device: 7.5 T superconducting, 11-pole wiggler

Optics

  • Vertical collimating mirror
  • Monochromator, either multilayer or Si(111) channel cut crystal
  • Toroidal focusing mirror

Beam Characteristics

With Si(111) Channel Cut Crystal Monochromator

  • Energy range: 7.0 - 17.5 keV  (1.77 Å - 0.71 Å)
  • Bandpass: Δλ/λ: 2.0x10-4
  • Spot size, horizontal x vertical (FWHM): 1.6 mm x 0.4 mm

With Multilayer Monochromator   (not yet in service)

  • Energy range: 9 - 13 keV (1.38 Å - 0.95 Å)
  • Bandpass: Δλ/λ :
  • Spot size, horizontal x vertical (FWHM):

Station

  • Sample positioning: Mar Research dtb single-axis goniostat

Chi motion for crystal mounting (0 - 60°) Motorized sample alignment (Marxy) system

  • Sample to detector distance: 50 - 300 mm
  • Detector theta: 0° - 30°
  • Sample temperature control: Oxford Instruments CryoJet (100 K - room temperature)
  • Detector: Mar165 CCD detector
  • Fluorescence detector: Oxford Cyberstar

Other equipment/facilities available

  • Incubators (0 - 50° C)
  • Storage dewar
  • Small dewars
  • Hampton cryotools
  • Stereo microscopes   (with polarizer available)
  • Anaerobic chamber
  • Wet lab with fume hood, balances, centrifuges etc.

Computers and Software

The beamline has computers running Linux (RHEL and Centos) with the following crystallographic software:

  • CCP4
  • HKL-2000
  • Phenix
  • Coot

Data are stored on a Network Attatched Storage system confgured to provide 1 TB of RAID 1 (mirrored)  disk space.   This corresponds to about 120K uncompressed ccd images.

Both wired (100 base T) and WiFi connections are available for user's laptops.  

Data Backup

  • CD/DVD burners    Supporting both DVD+ and DVD- formats.
  • Portable Hard Drives and Data Sticks USB 2.0

  • External Interface for SATA Drives

The appropriate media should be provided by the user for data backup.